NA = 0.7 + 100x magnification, achieving a resolution of 0.4μm (theoretical maximum at 550nm), approaching the limit of optical microscopes; a working distance of 6mm requires strict light control (it is recommended to use a coverslip), and the extremely shallow depth of focus (±0.56μm) has extremely high requirements for the flatness of the sample.

It is applied to the observation of virus morphology (high staining degree), protein crystals, and semiconductor quantum dots.